Figure 2. Synergistic induction of SREBP-1c mRNA and protein by insulin and T0901317.

Primary hepatocytes were cultured for 23 h prior to addition of 30 nM insulin or 10 μM T0901317 or both together. At the indicated time points, cells were processed for isolation of total RNA or protein. (A) Time course of increase in SREBP-1c mRNA after addition of 30 nM insulin (□) or 10 μM T0901317 (○) or insulin plus T0901317 (△). mRNA levels were assayed by RT-qPCR. Results are the means±S.E.M. for four separate experiments. Note that the y-axis is discontinuous, with different scales in lower and upper parts of the plot. (B) Time-course of increase in 130 kDa SREBP-1c precursor (upper panel) and 69 and 73 kDa processed SREBP-1c (lower panel). The experiment was repeated three times with similar results. kd=kDa.