Figure 3. Differential effects of insulin and T0901317 on binding of SREBP-1c to the FASN and GCK promoters and induction of mRNA expression.

Hepatocytes were cultured for 23 h prior to addition of 30 nM insulin or 10 μM T0901317 or both together. The cells were assayed for binding of SREBP-1c to the FASN (A) and GCK (B) promoters by ChIP, 4 h after addition of the inducers. Quantification was performed as described in the Experimental procedures. Results are means±S.E.M. of four independent experiments. Total RNA was isolated from hepatocytes at the indicated time points. Relative FASN (C), GCK (D) and CYP7A1 (E) mRNAs levels are shown for hepatocytes stimulated with 30 nM insulin (□) or 10 μM T0901317 (○) or insulin plus T0901317 (△). The mRNA assays were performed by RT-qPCR. Results are means±S.E.M. for at least four separate experiments.