Figure 5. Additive effects of SB216763 and T0901317 on FASN mRNA expression without any change in GCK mRNA levels.

Hepatocytes were cultured for 23 h before the addition of 10 μM SB216763, 10 μM T0901317 or 30 nM insulin. Vehicle (DMSO) was added to control cells. The cells were lysed for isolation of RNA after 8 h of treatment. FASN (A) or GCK (B) mRNA levels were analysed using RT-qPCR. Results are means±S.E.M. for six separate experiments. The inset in panel (B) shows the lack of effect of SB216763 and T0901317 on GCK mRNA on a magnified scale.