Table 1. Kinetic and thermodynamic parameters for the binding and inhibition of bacterial ART activity by HNP1.
| Variable NAD+ substrate: uncompetitive inhibition | Variable eEF2 substrate: competitive inhibition | |||
|---|---|---|---|---|
| Toxin | IC50 (μM) | Ki (μM) | Kd (μM) | Ki (μM) |
| DT | 11.1±2.6 | 7.3±1.7 | 6.8±1.2 | 6.7±1.1 |
| ETA | 19.2±6.5 | 13.3±0.9 | 21.5±2.1 | 5.3±0.4 |