Table 1. Kinetic parameters for exocyclic DNA MTases.
HM, hemimethylated; L, linear; SC, supercoiled; UM, unmethylated.
| Enzyme | kcat (s−1) | Km(DNA) (M) | Km(AdoMet) (M) | kcat/Km(DNA) (M−1·s−1) | DNA substrate | Reference |
|---|---|---|---|---|---|---|
| M.EcoRV | 9.4×10−5 | 7.1×10−6 | 0.013×103 | 12 UM | [36] | |
| 20.3×10−5 | 1.0×10−6 | 0.203×103 | 16 UM | [36] | ||
| 27×10−5 | 0.3×10−6 | 0.9×103 | 20 UM | [36] | ||
| 16.7×10−5 | 0.91×10−6 | 0.184×103 | 20 HM | [36] | ||
| 44.7×10−5 | 0.27×10−6 | 1.66×103 | 382 UM | [36] | ||
| M.EcaI | 0.0047 | 182×10−9 | 1.8×10−6 | 2.58×104 | 24 UM | [28,37] |
| 0.0025 | 91×10−9 | 2.7×104 | 24 HM | [28] | ||
| M.EcoRI | 0.11 | 28×10−9 | 1.17×10−6 | 3.8×106 | 14 UM | [38] |
| 0.58 | 25×10−9 | 0.23×108 | 14 UM | [39] | ||
| 0.23 | 1.3×10−9 | 1.8×108 | pBR322 | [39] | ||
| 0.14 | 24.4×10−9 | 0.28×10−6 | 451×106 | 14 UM | [40] | |
| 0.105 | 42.9×10−9 | 0.248×10−6 | 2.45×106 | 14 HM | [40] | |
| 0.124 | 2.44×10−9 | 118×10−9 | 0.51×108 | 14 bp | [26] | |
| 0.142 | 0.346×10−9 | 210×10−9 | 4.1×108 | pBR322 | [26] | |
| 0.58 | 25×10−9 | 0.24×108 | 14 bp | [39] | ||
| 0.25 | 5.9×10−9 | 0.42×108 | 106 bp | [39] | ||
| 0.16 | 2.1×10−9 | 0.76×108 | 222 bp | [39] | ||
| 0.25 | 2.4×10−9 | 1.0×108 | 429 bp | [39] | ||
| 0.37 | 3.7×10−9 | 1.0×108 | 2326 bp | [39] | ||
| 0.30 | 3.2×10−9 | 0.94×108 | 4361 bp | [39] | ||
| EcoDam | 0.0041 | 122×10−9 | 33.7×103 | 14 UM | [41] | |
| 0.316 | 3.6×10−9 | 12.2×10−6 | 8.77×107 | ColEI DNA | [42] | |
| 0.0208 | 25.5×10−9 | 816×103 | 14 bp HM | [41] | ||
| 0.93 | 17.4×10−9 | 5.6×10−6 | 20 bp | [34] | ||
| T4 Dam | 0.015 | 6.3×10−9 | 2.38×106 | 20 bp UM | [43] | |
| 0.010 | 6.0×10−9 | 1.67×106 | 20 bp HM | [43] | ||
| 0.014 | 7.7×10−9 | 1.8×106 | 24 bp UM | [44] | ||
| 0.14 | 1.1×10−12 | 0.1×10−6 | 1.27×1011 | T4 gt− dam DNA | [45] | |
| M.BamHI | 0.0175 | 2.1×10−6 | 8.3×103 | 12 bp | [46] | |
| 0.053 | 196×10−9 | 1.22×10−6 | 2.7×105 | 20 bp UM | [23] | |
| M.EcoP15I | 0.0053 | 8×10−7 | 6.62×103 | 31 bp | [47] | |
| 9.6×10−5 | 8.3×10−7 | 1.15×102 | pUC 18 | [48] | ||
| M.TaqI (60 °C) | 0.73 | 0.6×10−6 | 3.7×10−6 | 1.21×106 | 36 HM | [49] |
| M.FokI | 4.5×10−4 | 1.6×10−7 | 2.81×103 | 21 UM | [50] | |
| 0.002 | 1.3×10−7 | 1.54×104 | 21 HM | [50] | ||
| M.ClaI | 5.5×10−5 | 1.3×10−6 | 42.3 | 12 UM | [51] | |
| CcrM | 3.3×10−4 | 45/50 HM | [29] | |||
| M.BcgI | 8.3×10−6 | 3.0×10−7 | 27.7 | λ DNA | [52] | |
| M.MvaI | 7.5×10−3 | 45×10−9 | 1.67×105 | 14 bp | [53] | |
| M.KpnI | 3.3×10−4 | 0.15×10−6 | 2.2×103 | pUC18 DNA (SC) | [35] | |
| 7.2×10−4 | 0.09×10−6 | 8.0×103 | pUC 18 DNA (L) | |||
| 2.2×10−3 | 1.95×10−6 | 1.1×103 | UM | |||
| 5.6×10−3 | 1.42×10−6 | 4.0×103 | HM | |||
| M.RsrI | 1.9×10−2 | − | − | − | − | [54] |