Table 2. Substrate (DNA and AdoMet)-binding affinities for DNA MTases.

K_d values for DNA reported were determined in the presence of cofactor; (−) indicates the binding constants determined in the absence of cofactor (AdoHcy or sinefungin); (+) with cofactor. HM, hemimethyltated; NSP, non-specific DNA; SP, specific DNA; UM, unmethylated.

Enzyme	Kd(DNA) (M)	Kd(AdoMet) (M)	DNA substrate	Reference
M.EcoRV	46×10−9		30 UM	[60]
	11×10−9		30 HM
	143×10−9		30 DM
M.EcoRI		30×10−6	14 bp	[26]
		10×10−6		[38]
	0.43		14 bp	[39,61]
	0.29		32 bp
	0.10		101 bp
	0.07		378 bp
	0.02		775 bp
	4.4×10−9		14 UH	[62]
	4.1×10−9		14 HM
	(1–4)×10−6		pBR322	[26]
EcoDam	(10–20)×10−9		14 bp SP	[41]
	(10–20)×10−9		14 bp NSP
	(60–70)×10−9 (+)	6.31×10−6	14 bp SP
	(300–400)×10−9 (−)		14 bp SP	[56]
	1.6×10−6		14 bp NSP
	(3–4)×10−6 (−)		14 bp NSP
	119×10−9		20 bp	[34]
	3.4×10−6		20 bp	[63]
T4 Dam	16.5×10−9		20 bp UM	[43]
	8.5×10−9		20 bp HM
	18×10−9 (+)		24 bp	[44]
	54×10−9 (−)
		7.6×10−6	T4 DNA	[45]
	8.57×10−9		14 bp SP	[64]
	3.3×10−9		14 bp NSP
M.EcoKI	1.6×10−9	3.6×10−6	45 bp UM	[58]
(M2S1)	9.1×10−9 (−)	2.21×10−6
	2.36×10−9 (−)		21 bp	[65]
	0.6×10−9
M1S1	13.97×10−9
	14.67×10−9 (−)
M.EcoP15I	1.0×10−6		31 bp	[47]
M.TaqI		2×10−6		[66]
M.RsrI		6.1×10−6	14 HM	[54]
	17 (+)		UM
	311 (−)		UM
	6 (+)		HM
	17 (−)		HM
	>800 (±)		NSP
M.KpnI		4.1×10−6		[35]
M.BamHI	371×10−9 (−)	1.22×10−9		[23]
	200×10−9 (+)