Table 2. Stern–Volmer constants for acrylamide, KI and CsCl quenching of trapped complexes of Pgp formed from ATP or ADP in the presence of Co2+ ions.
Two independent experiments were carried out for determination of each value of KSV, with different preparations of Pgp and the three trapped complexes in each case. The results are expressed as the means±range. NL, non-linear.
| KSV | |||
|---|---|---|---|
| Acrylamide | KI | CsCl | |
| Native Pgp | 1.40±0.012 | 1.17±0.036 | 0.343±0.019 |
| Pgp+Vi+ATP | 8.85±0.142 | 0.82±0.026 | 0.616±0.023 |
| Pgp+Vi+ADP | 2.49±0.019 | 0.71±0.030 | 0.355±0.015 |
| Pgp+BeFx+ATP | 2.88±0.032 | 0.96±0.038 | 0.275±0.010 |
| Pgp+BeFx+ADP | 2.05±0.025 | 0.99±0.013 | 0.101±0.008 |
| Pgp+AlFx+ATP | 1.37±0.066 | 1.09±0.080 | 0.262±0.014 |
| Pgp+AlFx+ADP | 2.34±0.079 | 0.83±0.032 | NL |
| NATA | 31.0±1.17 | 11.2±0.26 | 2.468±0.041 |