Skip to main content
. 2006 Sep 27;399(Pt 2):285–295. doi: 10.1042/BJ20060659

Figure 4. The DR-1 element of FAS is required for full glucose responsiveness.

Figure 4

(A) Schematic representation of the reporter constructs used in the subsequent luciferase assays. (B) COS-1 cells were transfected with the listed FAS luciferase reporter constructs and empty vector (open bars) or vector expressing HNF-4α (solid bars). Data are normalized to the luciferase activity of the luciferase constructs in the presence of vector alone, which is equal to 1. The results shown represent the means±S.E.M. for four independent experiments with three replicate transfections per experiment. (C) COS-1 cells were transfected with the listed FAS/L-PK luciferase reporter constructs as outlined in (B). (D) Rat primary hepatocytes were transfected with the listed luciferase reporter constructs. Cells were cultured in 5.5 mM (open bars) or 27.5 mM glucose (solid bars) for 48 h in the presence of 100 nM insulin and 100 nM dexamethasone. The data are expressed as fold increase in luciferase activity over 5.5 mM glucose. The results shown represent the means±S.E.M. for four independent experiments with three replicate transfections per experiment.