Table 1. Concentrations of substrates required for activation of ATPase activity of TM7 cysteine mutants by 50% (S50).
ND, not determined because of very low expression.
| Mutant | Verapamil S50 (μM) | Vinblastine S50 (μM) | Colchicine S50 (μM) |
|---|---|---|---|
| F711C | 9.8±1.1 | 2.3±0.2 | 410±20 |
| V712C | 10.0±0.8 | 1.9±0.2 | 320±40 |
| V713C | 9.9±1.3 | 1.8±0.2 | 340±40 |
| G714C | 14.1±2.1 | 1.9±0.3 | 410±30 |
| V715C | 9.8±1.3 | 1.8±0.3 | 330±40 |
| F716C | 10.1±1.0 | 2.1±0.1 | 340±30 |
| C717C | 10.0±1.5 | 2.3±0.3 | 390±20 |
| A718C | 10.5±1.3 | 2.0±0.1 | 330±30 |
| I719C | 10.5±1.5 | 1.9±0.2 | 370±30 |
| I720C | 12.4±0.7 | 2.4±0.2 | 390±10 |
| N721C | 13.0±1.2 | 1.7±0.2 | 380±40 |
| G722C | ND | ND | ND |
| G723C | 11.1±0.4 | 2.0±0.3 | 410±20 |
| L724C | 12.0±1.5 | 2.4±0.3 | 340±30 |
| Q725C | 11.7±1.1 | 2.0±0.1 | 390±20 |
| P726C | 11.9±1.0 | 1.9±0.2 | 450±30 |
| A727C | 21.2±3.1 | 1.9±0.2 | 480±40 |
| F728C | 48.7±2.2 | 2.7±0.3 | 830±90 |
| A729C | 34.3±3.0 | 2.3±0.2 | 760±80 |
| I730C | 12.0±1.1 | 2.0±0.1 | 420±30 |
| I731C | 13.5±1.9 | 1.9±0.2 | 410±30 |
| Cys-less | 11.6±1.3 | 2.1±0.3 | 400±40 |