TABLE 1.
sequences of LAH4 isomers, derivatives, and magainin 2 used in this study and their MIC50s for two model bacteria
| Peptidea | Sequenceb | Nominal charge at pH 5.5c | MIC (μM)
|
|||
|---|---|---|---|---|---|---|
|
E. coli
|
B. megaterium
|
|||||
| pH 7.2 | pH 5.5 | pH 7.2 | pH 5.5 | |||
| LAH4-L0 | KKALLAHALAHLALLALHLALHLKKA | +9 | 5 | 0.5 | 5 | 1 |
| LAH4-L1 | KKALLAHALHLLALLALHLAHALKKA | +9 | 5 | 0.5 | 5 | 5 |
| LAH4-L2 | KKALLALALHHLALLALHLAHALKKA | +9 | 5 | 0.5 | 5 | 5 |
| LAH4-AL6 | KKALLHLALALLALHAHALALHLKKA | +9 | 5 | 0.5 | 5 | 1 |
| LAH4-L1-F4 | KKALLAHFFHLLALLALHFFHALKKA | +9 | 20 | 0.5 | 10 | 5 |
| LAH6-L1 | KKALLAHALHHLALLAHHLAHALKKA | +11 | 1 | 0.5 | 1 | 1 |
| Magainin 2 | GIGKFLHSAKKFGKAFVGEIMNS | +5 | 15 | 0.5 | 10 | 5 |
a
The peptides are all C-terminally amidated, contributing one extra positive charge overall.
b
Histidine residues in the peptides are marked in bold.
c
At neutral pH, the LAH peptides and magainin 2 have nominal charges of +5 and +4, respectively.