FIG. 2.

Cellular retention of [³H]T16 in hematozoan-parasitized (•) or unparasitized (○) erythrocytes. (A) B. divergens- and (B) P. falciparum-parasitized erythrocytes or control unparasitized erythrocytes were preloaded with [³H]T16 (50 nM for 2 h at 37°C) and then washed and resuspended in complete culture medium with (dashed lines) or without (solid lines) 200 nM of nonradioactive T16. Plots show [³H]T16 remaining in the erythrocytes at the specified times. [³H]T16 cellular retention at 4°C in parasitized erythrocytes (▴) is also shown. Data are the means ± standard errors of the means of three independent experiments (small standard errors of the means are hidden by the symbols), each measure being performed in triplicate. For P. falciparum-infected erythrocytes, cellular retention of preaccumulated [³H]T16 at each time point was significantly altered by the presence of 200 nM external nonradioactive T16 (P < 0.1, Student's t test).