FIG. 4.

Distribution on discontinuous sucrose gradients of the accumulated compounds after incubation with 50 nM of T16 or CQ for 2 h at 37°C. B. divergens-infected erythrocytes were incubated with [³H]T16 (solid bars), and P. falciparum erythrocytes were incubated with [³H]T16 (hashed bars) or [³H]chloroquine (blank bars). Parasitized erythrocytes were then permeabilized with streptolysin O, and freed parasites collected by centrifugation. (A) SLO-freed parasites were directly overlaid onto discontinuous sucrose gradients (depicted schematically at the left of the figure). (B) Streptolysin O-freed parasites were sonicated and overlaid onto gradients. Each value is the means ± standard errors of the means of three independent experiments, each measure being performed in triplicate.