FIG. 4.

Luciferase activity in 293 cells infected with IN-competent (squares; left y axes) or IN-defective (diamonds; right y axes) luciferase-expressing self-inactivating lentiviral vectors. The cells were infected in a 96-well plate format with 1 cpm/cell equivalent/well and treated with increasing amounts of the anti-IN drug L-731,988 (range, 0.5 to 50 μM) (a) and the RT inhibitors AZT (range, 10 to 1,000 μM) (b) and ddI (range, 0,0005 to 10 μM) (c). Three days posttransduction, cell-associated luciferase activity was evaluated on a 96-well white plate with a clear bottom, using the Britelite Ultra-High Sensitivity Luminescence Reporter Gene Assay System. RLU, relative light units. Each condition was tested in three replicates, and mean values with standard deviations are shown.