FIG. 1.

Sensitivities of C. albicans strains to CFW, SDS, and nikkomycin Z. The strains are indicated above the lanes. All strains are prototrophic derivatives of the Arg⁻ Ura⁻ His⁻ strain BWP17 (4). DAY286 is a Ura⁺ Arg⁺ derivative of BWP17 that was transformed by a plasmid carrying RIM101SL and HIS1. (A) Droplets (3.5 μl) at two dilutions (10⁵ and 10⁴ cells/ml) were spotted on yeast extract-peptone-dextrose (YPD) medium and YPD medium supplemented with 20 μg/ml CFW (Sigma; fluorescent brightener 28) or 0.05% SDS. The plates were incubated at 30°C for 48 h, except for the plates with 0.05% SDS, which were incubated for 5 days. (B) Droplets (3.5 μl) at two dilutions (10⁵ and 10⁴ cells/ml) were spotted on synthetic complete medium (3) and synthetic medium supplemented with 8 μg/ml of nikkomycin Z (Sigma). The plates were incubated at 30°C for 48 h.