TABLE 1.
Candida albicans susceptibilitiesa
| Strain | Geometric mean MIC (μg/ml)b
|
|||
|---|---|---|---|---|
| Fluconazole | Voriconazole | Caspofungin | Micafungin | |
| ATCC 90028 | 0.96 | 0.03 | 0.05 | 0.06 |
| DAY185 (control strain) | 0.62 | 0.02 | 0.06 | 0.03 |
| vps28−/− | 0.39 | 0.01 | 0.02 | 0.01 |
| vps32−/− | 0.32 | 0.01 | 0.02 | 0.01 |
| vps28−/−+VPS28 | 0.62 | 0.03 | 0.03 | 0.04 |
| vps32−/−+VPS32 | 0.68 | 0.02 | 0.03 | 0.04 |
| DAY286+RIM101SL | 0.75 | 0.03 | 0.04 | 0.02 |
| vps28−/−+RIM101SL | 0.75 | 0.02 | 0.007 | 0.001 |
| vps32−/−+RIM101SL | 0.62 | 0.01 | 0.005 | 0.001 |
| rim101−/− | 0.11 | 0.002 | 0.005 | 0.001 |
| rim101−/−+RIM101SL | 0.42 | 0.01 | 0.03 | 0.03 |
a
Calculated from at least 10 independent experiments. All strains derive directly from BWP17 (His− Ura− Arg−) (4) and are prototrophic. Double disruptions restored a Ura+ Arg+ phenotype. The His+ information was brought by the plamsids carrying VPS28, VPS32, or RIM101SL or by an empty vector, all integrated at HIS1. DAY286 is an Arg+ Ura+ derivative of DAY185.
b
Results are MIC minus 2 (prominent growth reduction or a 50% reduction in optical density) at 24 h for CAS and MCF and at 48 h for FLC and VRC.