TABLE 3.
Genes overexpressed at least 1.5-fold in different S. aureus strains after treatment with super-MICs of cell wall inhibitors
| N315 ORF | Gene | Product or putative function | Fold change (reference)a
|
|||
|---|---|---|---|---|---|---|
| COL (2× MIC of oxacillin) (present study) | JH1 (8× MIC of vancomycin) (16) | N315 (10× MIC of vancomycin) (14) | RN450 (super-MICs of oxacillin, bacitracin, and d-cycloserine) (31) | |||
| SA1712b | Conserved hypothetical protein | 4.64 | Up | 4.73 | Up | |
| SA2221b | Conserved hypothetical protein | 4.19 | 9.5 | 5.37 | 6.2-18.7 | |
| SA2113b | Conserved hypothetical protein | 3.90 | 10.4 | 2.65 | ||
| SA1659b | prsA | Parvuli-like peptidyl-prolyl isomerase | 3.80 | 12 | 5.74 | 2.7-7.7 |
| SA1701b | vraR | Two-component response regulator | 3.73 | 8.7 | 5.18 | Up |
| SA1700b | vraS | Two-component sensor histidine kinase | 3.75 | 8.7 | 7.41 | |
| SA2103b | Similar to lyt divergon expression attenuator LytR | 3.54 | 3.4 | 4.06 | 4.6-14 | |
| SA0914b | Similar to chitinase B | 3.39 | 10.6 | 1.94 | 4.9-7.6 | |
| SA1702b | yvqF | Conserved hypothetical protein | 2.91 | 8.1 | 7.27 | 3.4-7.8 |
| SA0591 | Conserved hypothetical protein | 2.82 | Up | Up | ||
| SA1549b | htrA | Heat shock protein homolog, similar to serine protease HtrA | 2.67 | 6.8 | 3.62 | 4.2-11 |
| SA1255b | PTS system, glucose-specific enzyme II, A component | 2.66 | 2.95 | 5.1-9.2 | ||
| SA1253b | ctpA | Carboxyl-terminal processing proteinase | 2.60 | 2.64 | ||
| SA1195 | msrR | Peptide methionine sulfoxide reductase regulator MsrR | 2.56 | 4.8 | 2.5-4.3 | |
| SA1283b | pbpB | Penicillin binding protein 2 | 2.42 | 1.87 | 2.8-4.9 | |
| SA2220b | Glycerate kinase | 2.36 | Up | 3.33 | 9.2-16.5 | |
| SA2146b | tcaA | TcaA protein | 2.32 | 4.1 | 2.40 | Up |
| SA2481 | Conserved hypothetical protein | 2.22 | 3.4 | 2.6-5.7 | ||
| SA2296b | Hypothetical protein, similar to transcriptional regulator MerR | 2.17 | Up | 3.87 | ||
| SA0824 | Conserved hypothetical protein | 2.17 | 5.8 | |||
| SA2343b | Hypothetical protein | 2.09 | Up | 6.55 | Up | |
| SA1925 | Conserved hypothetical protein | 2.00 | 3.2 | |||
| SA1703b | Hypothetical protein | 1.99 | 6.5 | 7.27 | Up | |
| SA0826 | spsB | Type 1 signal peptidase 1B | 1.81 | 4.7 | Up | |
| SA0222 | coa | Staphylococcal coagulase precursor | 1.74 | 3.17 | ||
| SA1691b | mgtB | Monofunctional glycosyltransferase | 1.69 | 10.6 | 5.64 | 5.2-10.9 |
| SA1926b | murZ | UDP-N-acetylglucosamine enolpyruvyl transferase | 1.63 | 5.4 | 2.52 | 3.1-8.9 |
| SA1711b | Hypothetical protein, similar to DNA damage-inducible protein P | 1.56 | 3.07 | |||
| SA2297b | Hypothetical protein, similar to GTP-pyrophosphokinase | 1.5 | 8.6 | 3.85 | 2.6-4.0 | |
a
Fold change refers to expression increases for up-regulated genes. Up, upregulated, numerical value not provided.
b
Member genes of the S. aureus VraSR regulon.