FIG. 2.

Inhibition of PRO 140-PE binding to CEM.NKR-CCR5 cells by unlabeled PRO 140, maraviroc, and vicriviroc. CEM.NKR-CCR5 cells were incubated with various concentrations of unlabeled PRO 140, maraviroc, or vicriviroc for 30 min at room temperature in PBSA buffer prior to the addition of 5 nM PRO 140-PE for an additional 30 min. Cells were washed and then analyzed by flow cytometry for both the MFI of binding and the percentage of cells gated for positive binding of PRO 140-PE. Inhibition was assessed on the basis of both MFI (A) and the percentage of cells gated (B).