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. 2006 Oct;72(10):6544–6553. doi: 10.1128/AEM.00749-06

FIG. 4.

FIG. 4.

Agarose gel electrophoresis of restriction endonuclease-digested pSL plasmid DNAs and Southern hybridization with drug resistance genes. (A) Agarose gel electrophoresis of endonuclease-digested pSL plasmid DNAs. Lanes: 1, EagI digestion; 2, EagI/XhoI double digestion; 3, XhoI digestion; 4, XhoI/FspI double digestion; 5, FspI digestion. The gels were run in triplicate and then Southern blotted and hybridized with a vanA probe (B), aph3 probe (C), or aac6/aph2 probe (D). The numbers on the left indicate the positions and sizes in kilobase pairs of the λ HindIII molecular size markers.