TABLE 4.
Transfer frequencies of pSL plasmids in Enterococcus
| Donor | Recipient | Transfer frequency (no. of transconjugants per donor cell)a |
|---|---|---|
| KV1 | E. faecium BM4105RF | <10−8 |
| KV1 | E. faecalis FA2-2 | 7 × 10−3 |
| KV2 | E. faecium BM4105RF | <10−8 |
| KV2 | E. faecalis FA2-2 | 4 × 10−3 |
| E. faecalis FA2-2 (pSL1) | E. faecalis JH2SS | 1 × 10−3 |
| E. faecalis FA2-2 (pSL2) | E. faecalis JH2SS | 2 × 10−3 |
| E. faecalis JH2SS (pSL1) | E. faecalis FA2-2 | 8 × 10−4 |
| E. faecalis JH2SS (pSL2) | E. faecalis FA2-2 | 7 × 10−4 |
a
Overnight cultures of 0.05 ml of donor and 0.45 ml of recipient were added to 4.5 ml of fresh N2GT, and the mixtures were incubated at 37°C with gentle agitation for 4 h. Portions of the mixed cultures were then plated on solid media with appropriate selective antibiotics. Colonies were counted after incubation for 48 h at 37°C.