TABLE 1.
Culturable population and phylogenetic characteristics of isolates from the black rust deposit on CORK 1026B
| Strain | MPN (cells per cm3) | Temp (°C) | Medium (reference) | Gas phase | Closest relative (% similarity)a |
|---|---|---|---|---|---|
| Methanogens | |||||
| Mc70 | 1.3 × 103 | 70 | MMJ (61) | 80% H2-20% CO2 (350 kPa) | Methanothermococcus thermolithotrophicus (99.5) |
| Mc55 | 1.3 × 103 | 55 | MMJ (61) | 80% H2-20% CO2 (350 kPa) | Methanothermococcus thermolithotrophicus (99.3) |
| Mc37 | 5.9 × 102 | 37 | MMJ (61) | 80% H2-20% CO2 (350 kPa) | Methanothermococcus thermolithotrophicus (99.1) |
| Ep55 | 5.9 | 55 | MMJHS (61) | 80% H2-20% CO2 (350 kPa) | Methanothermococcus thermolithotrophicus (99.5) |
| Ep70 | 5.9 | 70 | MMJHS (61) | 80% H2-20% CO2 (350 kPa) | Methanothermococcus thermolithotrophicus (99.4) |
| Sulfate reducers | |||||
| Tc37 | 49.6 | 37 | MJYPGS (41) | 100% N2 (200 kPa) | Desulfuromonas michiganensis (92.2) |
| Spi55 | 5.0 | 55 | MEtSR (this study)b | 80% N2-20% CO2 (250 kPa) | Desulfonatronovibrio hydrogenovorans (91.4) |
| Srb55 | 4.9 | 55 | MEtSR2 (this study)c | 80% H2-20% CO2 (350 kPa) | Desulfotomaculum geothermicum (96.1) |
| PM70-1 | NDh | 70 | DSMZ63-2 (this study)d | 80% H2-20% CO2 (200 kPa) | Archaeoglobus veneficus (95.1) |
| Fermenters | |||||
| Ag70 | 49.6 | 70 | MJYS (41) | 80% H2-20% CO2 (350 kPa) | Thermosipho melanesiensis (86.4) |
| Ag55 | 4.9 | 55 | MJYS (41) | 80% H2-20% CO2 (350 kPa) | Thermosipho melanesiensis (87.1) |
| Ag-C55 | 5.9 | 55 | MJYS2 (this study)e | 100% H2 (350 kPa) | Halothermothrix orenii (92.7) |
| Tc55 | 5.9 | 55 | MJYPGS (41) | 100% N2 (200 kPa) | Bacillus sp. strain BR (98.6) |
| Bal55 | 2.0 | 55 | MEtSR (this study) | 80% N2-20% CO2 (250 kPa) | Desulfotomaculum salinum (91.5) |
| Kimo37 | 2.0 | 37 | MJYP (50) | 100% N2 (200 kPa) | Cytophaga sp. strain AN-BI4 (88.6) |
| Yos55 | 2.0 | 55 | MJFM (this study)f | 80% N2-20% CO2 (250 kPa) | Thermohalobacter berrensis (91.5) |
| TH70-3 | ND | 70 | MSTH (this study)g | 100% N2 (200 kPa) | Thermosipho atlanticus (98.1) |
Determined by using the FASTA program of the DDBJ.
MEtSR contained 10 mM ethanol, 0.1 g yeast extract, 1 g NaHCO3, 1.5 g Na2SO4, 0.5 g Na2S, and 1 mg resazurin in 1 liter of MJ synthetic seawater (49).
MEtSR2 contained 0.5 g acetate, 0.5 g lactate, 0.5 g pyruvate, and 0.5 g, citrate instead of ethanol in 1 liter of MEtSR medium.
DSMZ63-2 was DSMZ medium 63 containing 2.5% Fe(NH4)2(SO4)2 instead of FeSO4.
MJYS2 was MJYS medium containing no NaHCO3.
MJFM contained 0.5 g yeast extract, 0.5 g peptone, 0.5 g formate, 0.5 g lactate, 0.5 g acetate, 0.5 g glucose, 0.5 g maltose, and 0.5 g citrate in 1 liter of MJ synthetic seawater (49).
MSTH contained 1 g yeast extract, 1 g peptone, 1 g NaHCO3, 0.5 g Na2S, and 1 mg resazurin in 1 liter of marine medium (70) amended with SL-10 trace mineral solution (71).
ND, not determined.