TABLE 1.
Relative abundances of 16S rRNA gene sequences of actively growing bacteria represented in T-RFLP profiles and clone libraries
| No. of bp before/after dredging | Closest match (NCBI accession no.) | % Identity | Relative abundance (%)
|
|
|---|---|---|---|---|
| T-RFLP profilea | Clone libraryb | |||
| Before | ||||
| 73 | VAS6, Corynebacterium sp. (AY592796) | 100 | 8.5 | 10.0 |
| 122 | VAS10, Prochlorococcus sp. (NC005072) | 98 | 3.8 | 4.8 |
| 194 | VAS9, Nitrobacter sp. (CP000115) | 97 | 1.8 | 2.9 |
| 215 | VAS14, Arthrobacter sp. (DQ341426) | 99 | 5.5 | 0.5 |
| 222 | VAS4, Spirochaeta sp. (AB121100) | 99 | 14.0 | 10.5 |
| 224 | VAS7, γ-proteobacterium (AY539822)c | 99 | 10.4 | 14.8 |
| 319 | VAS3, Flavobacterium sp. (AM167564) | 98 | 3.2 | 3.3 |
| 373 | VAS11, uncultured Cyanobacterium (AP008231) | 95 | 1.0 | 4.8 |
| After | ||||
| 71 | VAS25, Flavobacterium sp. (DQ007435) | 98 | 5.8 | 4.5 |
| 161 | VAS15, α-proteobacterium (DQ336968) | 99 | 1.6 | 5.9 |
| 173 | VAS21, Actinobacterium (UAC575535) | 97 | 5.1 | 6.8 |
| 203 | VAS23, uncultured bacterium (AB234247) | 99 | 2.4 | 4.5 |
| 293 | VAS16, Sphingomonas sp. (AF385529) | 100 | 14.6 | 17.8 |
a
Data represent the average relative abundance values of TRFs from three replicate T-RFLP profiles. The standard deviation for TRF replicates was ±0.4 to 1.2%.
b
rRNA gene sequences were digested in silico using the recognition site for restriction enzyme HaeIII.
c
This bacterium also was cultivated on PAH agar medium.