Infection dynamics of the spiroplasma during the developmental course of D. hydei strain TKB163. (A) Infection titers of the spiroplasma in terms of symbiont dnaA gene copies per insect. (B) Infection densities of the spiroplasma in terms of symbiont dnaA copies per host COII gene copy. Filled circles, adult females; open triangles, adult males; open circles, larvae and pupae without sexing. Mean values and standard deviations are shown. Sample sizes (female/male) are given in parentheses. The asterisks indicate statistically significant differences between males and females (Mann-Whitney U test: *, P < 0.05; **, P < 0.01; ***, P < 0.001).