TABLE 1.
Interspecific transfer of the non-male-killing spiroplasma from D. hydei to D. melanogastera
| Injection set | No. emerged (no. positive)b in generation:
|
|||||
|---|---|---|---|---|---|---|
| G1
|
G2
|
G3
|
||||
| Females | Males | Females | Males | Females | Males | |
| 1 | 9 (3/3) | 5 | ||||
| 2 | 6 (3/3) | 10 | 16 (2/2) | 28 | 4 (0/2) | 5 |
| 11 (0/8) | 9 (0/1) | |||||
| 3 | 17 (3/3) | 21 | 5 | 12 | ||
| 17 | 12 | |||||
| 4 | 33 (3/3) | 28 | 14 | 25 | ||
| 5 | 18 (3/3) | 23 | 16 (1/1) | 27 | ||
| 24 (9/9) | 29 | |||||
| 49 (8/8) | 43 | 11 (0/4) | 14 (5/5) | |||
| 18 (1/5) | 11 (3/5) | |||||
| 6 | 13 (3/3) | 15 | 11 | 14 | ||
| 20 | 31 | |||||
a
Hemolymph of the spiroplasma-infected strain of D. hydei (TKB163) was injected into adult females of the uninfected strain of D. melanogaster (Oregon-R), and their offspring were examined. In each of the generation columns, a brood produced by a single female is indicated in a single line.
b
For example, 9 (3/3) indicates that nine female adults emerged in the isofemale line and three of three adult females examined by diagnostic PCR were spiroplasma positive.