TABLE 2.

Microbial richness of the sample^a

OTU boundary (%)b	Statistic	Detected richness of the sample	Estimate of the total sample's richness
			Parametric model				Nonparametric estimator
			Inverse Gaussian	Lognormal	Pareto	2-mixed exponential	ACE1c	ACE1d
99	No. of OTUs	107	509	340	290	398	311	499
	SE	244	170	74	156	84	394
	Naïve GOF	0.30	0.17	0.10	0.11	NP
	Asymp. GOF	0.27	0.16	0.11	0.17
	τ	10	10	10	31	10	31
98	No. of OTUs	99	285	223	191	263	228	370
	SE	95	74	19	74	54	253
	Naïve GOF	0.19	0.26	0.12	0.29	NP
	Asymp. GOF	0.71	0.33	0.15	0.38
	τ	12	10	10	31	10	31
97	No. of OTUs	91	198	182	176	230	186	297
	SE	48	53	19	65	43	181
	Naïve GOF	0.39	0.30	0.21	0.12	NP
	Asymp. GOF	0.59	0.49	0.28	0.49
	τ	9	9	9	31	9	31
96	No. of OTUs	85	190	173	160	244	173	244
	SE	52	56	17	100	40	123
	Naïve GOF	0.13	0.09	0.07	0.11	NP
	Asymp. GOF	0.21	0.16	0.04	0.32
	τ	9	9	9	31	9	31
95	No. of OTUs	81	164	153	140	215	156	212
	SE	40	46	16	84	35	104
	Naïve GOF	0.26	0.19	0.15	0.15	NP
	Asymp. GOF	0.23	0.19	0.06	0.32
	τ	9	9	9	31	9	31
90	No. of OTUs	50	99	92	76	178	98	106
	SE	34	27	10	185	31	34
	Naïve GOF	0.06	0.05	0.04	0.12	NP
	Asymp. GOF	0.43	0.36	0.13	0.15
	τ	11	11	11	32	11	32
80	No. of OTUs	21	56,976	83	45	221	60	203
	SE	131,990	123	10	467	35	181
	Naïve GOF	0.02	0.01	0.02	0.00	NP
	Asymp. GOF	NA	NA	NA	NA
	τ	5	5	5	32	5	32

^aBoldfaced values represent selected estimates. τ, maximum frequency used in statistical procedure. Naïve GOF, uncorrected chi-square GOF P value; Asymp. GOF, asymptotically correct chi-square GOF P value; NA, not available; NP, computation not possible.

^bCutoff value for percent sequence similarity.

^cFor default τ.

^dFor maximum τ allowed by parametric model fit.