TABLE 2.
Proteins with altered level of synthesis in the luxS mutant PL2102
| plu no. | Function | Gene | Theoretical isoelectric point | Theoretical molecular mass (kDa) | Sequence coverage (%) | Induction ratioa (wild type/luxS mutant) |
|---|---|---|---|---|---|---|
| plu0075 | Superoxide dismutase | sodA | 6.52 | 23.5 | 39 | >3,000 |
| plu0158 | Putative l-arginine:lysine amidinotransferase | 5.43 | 42.2 | 38 | >800 | |
| plu0373 | Hemolysin-coregulated protein | 5.91 | 19.0 | 22 | 0.45 | |
| plu0375/plu3556 | Glutathione oxidoreductase/putative aminomethyltransferase | gor/- | 6.24/6.17 | 49.5/36.3 | 35/16 | 3.80 |
| plu0431 | Elongation factor G | fusA | 4.78 | 77.7 | 25 | 0.37 |
| plu0495 | Phosphoribosylaminoimidazolecarboxamide formyltransferase and IMP cyclohydrolase | purH | 6.16 | 57.7 | 48 | 3.75 |
| plu0565 | Threonine synthase | thrC | 4.91 | 47.8 | 32 | 0.50 |
| plu0692 | Prolyl-tRNA synthetase | proS | 5.15 | 63.7 | 42 | 2.25 |
| plu0831 | Beta-lactamase class C | ampC | 6.43 | 42.5 | 21 | 2.04 |
| plu0872 | 3-Methyl-2-oxobutanoate hydroxymethyltransferase | panB | 5.35 | 28.8 | 21 | 4.80 |
| plu0912 | CTP synthetase | pyrG | 6.14 | 60.2 | 27 | 11.60 |
| plu1174 | Iron compound ABC transporter substrate binding protein | 8.63 | 34.2 | 31 | >100 | |
| plu1253b | AI-2 production protein LuxS | luxS | 5.77 | 19.2 | 35 | Absent in luxS |
| plu1395/plu0375 | Cysteine synthase A/glutathione oxidoreductase | cysK/gor | 6.18/6.24 | 34.1/49.5 | 32/19 | >500 |
| plu1432 | Succinyl-CoA synthetase beta chain | sucC | 5.07 | 41.3 | 33 | >400 |
| plu1433 | Succinyl-CoA synthetase alpha chain | sucD | 6.25 | 29.9 | 26 | 3.37 |
| plu1750/plu2853 | Aspartate aminotransferase/solute binding periplasmic protein of iron-siderophore ABC transporter | aspC/- | 6.19/6.54 | 43.6/40.7 | 16/21 | 2.50 |
| plu1840 | Unknown | 4.67 | 40.0 | 25 | 2.04 | |
| plu2059 | Unknown | 4.30 | 54.8 | 20 | 4.21 | |
| plu2579 | Thiol peroxidase | tpx | 4.36 | 17.7 | 57 | 2.00 |
| plu2595 | Pyridoxamine kinase | pdxY | 6.23 | 31.7 | 61 | 2.08 |
| plu2665/plu3598 | Phenylalanyl-tRNA synthetase alpha chain/aminomethyltransferase | pheS/gcvT | 5.73/5.60 | 37.2/40.1 | 53/25 | 3.40/0.61 |
| plu2861 | Elongation factor P-like protein | 4.97 | 21.3 | 21 | 0.49 | |
| plu3147 | AI-2-processing aldolase | lsrF | 6.14 | 31.8 | 40 | 3.70 |
| plu3291 | Serine hydroxymethyltransferase | glyA | 6.32 | 45.3 | 33 | 2.90 |
| plu3621 | Dihydrolipoamide dehydrogenase | lpdA | 6.07 | 50.5 | 29 | 5.55 |
| plu3622 | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (E2) | aceF | 4.85 | 56.8 | 21 | 3.73 |
| plu3673 | 2-Isopropylmalate synthase | leuA | 5.52 | 57.5 | 24 | >1,000 |
| plu3739b | Aldehyde dehydrogenase B | aldB | 5.28 | 54.2 | 46 | 0.47 |
| plu3795 | Unknown | 4.72 | 16.5 | 62 | 2.08 | |
| plu3870b | Trigger factor (chaperone) | tig | 4.46 | 48.6 | 29 | 0.33 |
| plu3907 | Alkyl hydroperoxide reductase small subunit (antioxidant) | ahpC | 6.40 | 22.3 | 64 | 2.60 |
| plu4064 | Putative modulator of DNA gyrase | tldD | 5.10 | 51.4 | 42 | 0.50 |
| plu4098 | Leucine-specific binding protein precursor | livK | 6.79 | 39.6 | 35 | 0.30 |
| plu4130 | Elongation factor P | efp | 4.58 | 20.7 | 27 | 5.00 |
| plu4134 | 60-kDa chaperonin | groEL | 4.60 | 57.5 | 23 | 0.50 |
| plu4332 | Alcohol dehydrogenase class III | adhC | 6.23 | 39.3 | 28 | <300 |
| plu4547b | Malate dehydrogenase | mdh | 5.50 | 32.6 | 46 | >500 |
| plu4730 | Elongation factor TufA | tufA | 4.90 | 43.2 | 37 | 3.20 |
| plu4742 | Arginosuccinate synthase | argG | 5.36 | 44.79 | 36 | 2.02 |
| plu4871 | 87% similarity with alpha helix protein YicC of E. coli | 4.78 | 33.6 | 32 | 0.50 |
a
Induction ratios were calculated from the mean of three values after normalization. In the normalization method used, the raw quantity of each spot in a member gel is divided by the total intensity value of all the pixels in the image.
b
Gene found in the equivalent transcriptome experiment.