Skip to main content
. 2006 Oct;72(10):6743–6750. doi: 10.1128/AEM.00584-06

FIG. 2.

FIG. 2.

Distribution of eGFP fusion of perilipin A in recombinant R. opacus. The left panels shows phase-contrast images, whereas the right panels shows the corresponding fluorescence images. (A) A pJAM2-egfp-transformed cell of R. opacus grown for 24 h under storage conditions shows a diffuse cytoplasmic fluorescence of unfused eGFP. The arrow indicates an area excluded from fluorescence due to an intracellular, unmarked TAG inclusion. (B) Cells transformed with pJAM2-perAmur-egfp grown for 0, 24, or 48 h under storage conditions, expressing eGFP-fused murine perilipin A. Fluorescence of the eGFP fusion is associated with intracellular TAG inclusions (arrow). (C) TAG inclusion isolated from a perilipin A-eGFP-expressing R. opacus cell grown for 48 h under storage conditions. After isolation of the TAG inclusion, counterstaining of core lipids was performed with Nile Red.