TABLE 1.
Strains and plasmids used
| Strain or plasmid | Description, origin, or sequence | Source or reference |
|---|---|---|
| P. putida strains | ||
| KT2440 | mt-2 hsdR1 (r− m+) | 1 |
| KTaauR | aauR derivative of KT2440 | This work |
| KTaauS | aauS derivative of KT2440 | This work |
| KTansB::lacZ | KT2440 containing the PP2453 promoter region fused to a promoterless lacZ gene | This work |
| KTABC::lacZ | KT2440 containing the PP1071 promoter fused to a promoterless lacZ gene | This work |
| KTaauRansB::lacZ | KTaauR containing the PP2453 promoter fused to a promoterless lacZ gene | This work |
| KTaauRABC::lacZ | KTaauR containing the PP1071 promoter fused to a promoterless lacZ gene | This work |
| E. coli strains | ||
| HB101 | supE44 hsdS20 (rB− mB−) recA13 ara-14 proA21 acY1 galK2 rpsL20 | Amersham Biosciences |
| S-17 | 15 | |
| Plasmids | ||
| pK18 | Cloning vector, kanamycin resistance | 12 |
| PDN19lacΩ | Promoterless lacZ oriV oriT Tetr Strr Ω fragment |