TABLE 2.
Characteristics of differentially expressed proteins in P. putida KT2440 and an isogenic aauR mutant during growth on glutamate and glucose-NH4+
| Growth conditionsc | Spot | Identification | No. of matching peptides | Locus | pIcalca | Masscalc (kDa)b | Function |
|---|---|---|---|---|---|---|---|
| Glutamate | R1 | Carboxyphosphonoenolpyruvate phosphonomutase | 13 | PP1389 | 5.26 | 31.9 | Unknown |
| R2 | Conserved hypothetical protein | 11 | PP3089 | 5.42 | 19.5 | Unknown | |
| R3 | Amino acid ABC transporter, periplasmic amino acid binding protein | 20 | PP1071 | 8.61 | 33.5 | Amino acid transport | |
| R4 | PGA | 22 | PP2453 ansB | 6.9 | 38.6 | Hydrolysis of Gln and Asn | |
| Glucose-NH4+ | D1 | Branched-chain amino acid ABC transporter, periplasmic amino acid binding protein | 15 | PP4867 | 5.93 | 40.3 | Amino acid transport |
| D2 | Branched-chain amino acid ABC transporter, periplasmic amino acid binding protein | 15 | braC (PP1141) | 6.02 | 39.4 | Amino acid transport | |
| D3 | General amino acid ABC transporter, periplasmic binding protein | 17 | aapJ (PP1297) | 5.84 | 36.4 | Amino acid transport |
a
pIcalc, calculated isoelectric point.
b
Masscalc, calculated mass.
c
Spots were downregulated during growth of the KTaauR mutant.