Figure 5.
Adult corneal stroma cells also formed nestin-expressing cell clusters. Stromal cells freshly isolated from 12-week-old mice formed similar cell clusters (A). DAPI expression (B). A cell cluster expressing nestin (C). Merged image showing the expression of nestin in the cluster and neuronlike elongated cells. Nestin was not expressed by the stromal cells (D). Cell clusters and migrating cells reacted against pgp 9.5 (E). Migrating cells but not the cell clusters reacted against tyrosine hydroxylase (F). Bar, 25 μm.