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. 1996 Oct;8(10):1833–1844. doi: 10.1105/tpc.8.10.1833

Mechanisms of Pathogen-Derived Resistance to Viruses in Transgenic Plants.

DC Baulcombe 1
PMCID: PMC161318  PMID: 12239365

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Selected References

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  1. Abel P. P., Nelson R. S., De B., Hoffmann N., Rogers S. G., Fraley R. T., Beachy R. N. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science. 1986 May 9;232(4751):738–743. doi: 10.1126/science.3457472. [DOI] [PubMed] [Google Scholar]
  2. Anderson J. M., Palukaitis P., Zaitlin M. A defective replicase gene induces resistance to cucumber mosaic virus in transgenic tobacco plants. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8759–8763. doi: 10.1073/pnas.89.18.8759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Audy P., Palukaitis P., Slack S. A., Zaitlin M. Replicase-mediated resistance to potato virus Y in transgenic tobacco plants. Mol Plant Microbe Interact. 1994 Jan-Feb;7(1):15–22. doi: 10.1094/mpmi-7-0015. [DOI] [PubMed] [Google Scholar]
  4. Baulcombe D. Replicase-mediated resistance: a novel type of virus resistance in transgenic plants? Trends Microbiol. 1994 Feb;2(2):60–63. doi: 10.1016/0966-842x(94)90128-7. [DOI] [PubMed] [Google Scholar]
  5. Beck D. L., Guilford P. J., Voot D. M., Andersen M. T., Forster R. L. Triple gene block proteins of white clover mosaic potexvirus are required for transport. Virology. 1991 Aug;183(2):695–702. doi: 10.1016/0042-6822(91)90998-q. [DOI] [PubMed] [Google Scholar]
  6. Bendahmane A., Köhn B. A., Dedi C., Baulcombe D. C. The coat protein of potato virus X is a strain-specific elicitor of Rx1-mediated virus resistance in potato. Plant J. 1995 Dec;8(6):933–941. doi: 10.1046/j.1365-313x.1995.8060933.x. [DOI] [PubMed] [Google Scholar]
  7. Brederode F. T., Taschner P. E., Posthumus E., Bol J. F. Replicase-mediated resistance to alfalfa mosaic virus. Virology. 1995 Mar 10;207(2):467–474. doi: 10.1006/viro.1995.1106. [DOI] [PubMed] [Google Scholar]
  8. Carr J. P., Gal-On A., Palukaitis P., Zaitlin M. Replicase-mediated resistance to cucumber mosaic virus in transgenic plants involves suppression of both virus replication in the inoculated leaves and long-distance movement. Virology. 1994 Mar;199(2):439–447. doi: 10.1006/viro.1994.1142. [DOI] [PubMed] [Google Scholar]
  9. Carrington J. C., Kasschau K. D., Mahajan S. K., Schaad M. C. Cell-to-Cell and Long-Distance Transport of Viruses in Plants. Plant Cell. 1996 Oct;8(10):1669–1681. doi: 10.1105/tpc.8.10.1669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Clark W. G., Fitchen J. H., Beachy R. N. Studies of coat protein-mediated resistance to TMV. I. The PM2 assembly defective mutant confers resistance to TMV. Virology. 1995 Apr 20;208(2):485–491. doi: 10.1006/viro.1995.1179. [DOI] [PubMed] [Google Scholar]
  11. Clark W. G., Fitchen J., Nejidat A., Deom C. M., Beachy R. N. Studies of coat protein-mediated resistance to tobacco mosaic virus (TMV). II. Challenge by a mutant with altered virion surface does not overcome resistance conferred by TMV coat protein. J Gen Virol. 1995 Oct;76(Pt 10):2613–2617. doi: 10.1099/0022-1317-76-10-2613. [DOI] [PubMed] [Google Scholar]
  12. Cooper B., Lapidot M., Heick J. A., Dodds J. A., Beachy R. N. A defective movement protein of TMV in transgenic plants confers resistance to multiple viruses whereas the functional analog increases susceptibility. Virology. 1995 Jan 10;206(1):307–313. doi: 10.1016/s0042-6822(95)80046-8. [DOI] [PubMed] [Google Scholar]
  13. Day A. G., Bejarano E. R., Buck K. W., Burrell M., Lichtenstein C. P. Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6721–6725. doi: 10.1073/pnas.88.15.6721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Donson J., Kearney C. M., Turpen T. H., Khan I. A., Kurath G., Turpen A. M., Jones G. E., Dawson W. O., Lewandowski D. J. Broad resistance to tobamoviruses is mediated by a modified tobacco mosaic virus replicase transgene. Mol Plant Microbe Interact. 1993 Sep-Oct;6(5):635–642. doi: 10.1094/mpmi-6-635. [DOI] [PubMed] [Google Scholar]
  15. Dorer D. R., Henikoff S. Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. Cell. 1994 Jul 1;77(7):993–1002. doi: 10.1016/0092-8674(94)90439-1. [DOI] [PubMed] [Google Scholar]
  16. English J. J., Mueller E., Baulcombe D. C. Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes. Plant Cell. 1996 Feb;8(2):179–188. doi: 10.1105/tpc.8.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Finnegan E. J., Peacock W. J., Dennis E. S. Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8449–8454. doi: 10.1073/pnas.93.16.8449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Golemboski D. B., Lomonossoff G. P., Zaitlin M. Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6311–6315. doi: 10.1073/pnas.87.16.6311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Green P. J. Control of mRNA Stability in Higher Plants. Plant Physiol. 1993 Aug;102(4):1065–1070. doi: 10.1104/pp.102.4.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hellwald K. H., Palukaitis P. Viral RNA as a potential target for two independent mechanisms of replicase-mediated resistance against cucumber mosaic virus. Cell. 1995 Dec 15;83(6):937–946. doi: 10.1016/0092-8674(95)90209-0. [DOI] [PubMed] [Google Scholar]
  21. Hobbs S. L., Kpodar P., DeLong C. M. The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol. 1990 Dec;15(6):851–864. doi: 10.1007/BF00039425. [DOI] [PubMed] [Google Scholar]
  22. Hobbs S. L., Warkentin T. D., DeLong C. M. Transgene copy number can be positively or negatively associated with transgene expression. Plant Mol Biol. 1993 Jan;21(1):17–26. doi: 10.1007/BF00039614. [DOI] [PubMed] [Google Scholar]
  23. Hong Y., Saunders K., Hartley M. R., Stanley J. Resistance to geminivirus infection by virus-induced expression of dianthin in transgenic plants. Virology. 1996 Jun 1;220(1):119–127. doi: 10.1006/viro.1996.0292. [DOI] [PubMed] [Google Scholar]
  24. Huntley C. C., Hall T. C. Minus sense transcripts of brome mosaic virus RNA-3 intercistronic region interfere with viral replication. Virology. 1993 Jan;192(1):290–297. doi: 10.1006/viro.1993.1032. [DOI] [PubMed] [Google Scholar]
  25. Ingelbrecht I., Van Houdt H., Van Montagu M., Depicker A. Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10502–10506. doi: 10.1073/pnas.91.22.10502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Kaido M., Mori M., Mise K., Okuno T., Furusawa I. Inhibition of brome mosaic virus (BMV) amplification in protoplasts from transgenic tobacco plants expressing replicable BMV RNAs. J Gen Virol. 1995 Nov;76(Pt 11):2827–2833. doi: 10.1099/0022-1317-76-11-2827. [DOI] [PubMed] [Google Scholar]
  27. Kakutani T., Jeddeloh J. A., Richards E. J. Characterization of an Arabidopsis thaliana DNA hypomethylation mutant. Nucleic Acids Res. 1995 Jan 11;23(1):130–137. doi: 10.1093/nar/23.1.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Kollàr A., Dalmay T., Burgyàn J. Defective interfering RNA-mediated resistance against cymbidium ringspot tombusvirus in transgenic plants. Virology. 1993 Mar;193(1):313–318. doi: 10.1006/viro.1993.1127. [DOI] [PubMed] [Google Scholar]
  29. Kumagai M. H., Donson J., della-Cioppa G., Harvey D., Hanley K., Grill L. K. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1679–1683. doi: 10.1073/pnas.92.5.1679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Lawson C., Kaniewski W., Haley L., Rozman R., Newell C., Sanders P., Tumer N. E. Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Biotechnology (N Y) 1990 Feb;8(2):127–134. doi: 10.1038/nbt0290-127. [DOI] [PubMed] [Google Scholar]
  31. Lindbo J. A., Dougherty W. G. Pathogen-derived resistance to a potyvirus: immune and resistant phenotypes in transgenic tobacco expressing altered forms of a potyvirus coat protein nucleotide sequence. Mol Plant Microbe Interact. 1992 Mar-Apr;5(2):144–153. doi: 10.1094/mpmi-5-144. [DOI] [PubMed] [Google Scholar]
  32. Lindbo J. A., Dougherty W. G. Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts. Virology. 1992 Aug;189(2):725–733. doi: 10.1016/0042-6822(92)90595-g. [DOI] [PubMed] [Google Scholar]
  33. Lindbo J. A., Silva-Rosales L., Proebsting W. M., Dougherty W. G. Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance. Plant Cell. 1993 Dec;5(12):1749–1759. doi: 10.1105/tpc.5.12.1749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Maiti I. B., Murphy J. F., Shaw J. G., Hunt A. G. Plants that express a potyvirus proteinase gene are resistant to virus infection. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6110–6114. doi: 10.1073/pnas.90.13.6110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Malyshenko S. I., Kondakova O. A., Nazarova JuV, Kaplan I. B., Taliansky M. E., Atabekov J. G. Reduction of tobacco mosaic virus accumulation in transgenic plants producing non-functional viral transport proteins. J Gen Virol. 1993 Jun;74(Pt 6):1149–1156. doi: 10.1099/0022-1317-74-6-1149. [DOI] [PubMed] [Google Scholar]
  36. Nicholson A. W. Structure, reactivity, and biology of double-stranded RNA. Prog Nucleic Acid Res Mol Biol. 1996;52:1–65. doi: 10.1016/s0079-6603(08)60963-0. [DOI] [PubMed] [Google Scholar]
  37. Osbourn J. K., Watts J. W., Beachy R. N., Wilson T. M. Evidence that nucleocapsid disassembly and a later step in virus replication are inhibited in transgenic tobacco protoplasts expressing TMV coat protein. Virology. 1989 Sep;172(1):370–373. doi: 10.1016/0042-6822(89)90143-8. [DOI] [PubMed] [Google Scholar]
  38. Pang S. Z., Slightom J. L., Gonsalves D. Different mechanisms protect transgenic tobacco against tomato spotted wilt and impatiens necrotic spot Tospoviruses. Biotechnology (N Y) 1993 Jul;11(7):819–824. doi: 10.1038/nbt0793-819. [DOI] [PubMed] [Google Scholar]
  39. Petty I. T., Jackson A. O. Mutational analysis of barley stripe mosaic virus RNA beta. Virology. 1990 Dec;179(2):712–718. doi: 10.1016/0042-6822(90)90138-h. [DOI] [PubMed] [Google Scholar]
  40. Prins M., Resende R. de O., Anker C., van Schepen A., de Haan P., Goldbach R. Engineered RNA-mediated resistance to tomato spotted wilt virus is sequence specific. Mol Plant Microbe Interact. 1996 Jul;9(5):416–418. doi: 10.1094/mpmi-9-0416. [DOI] [PubMed] [Google Scholar]
  41. Reimann-Philipp U., Beachy R. N. Coat protein-mediated resistance in transgenic tobacco expressing the tobacco mosaic virus coat protein from tissue-specific promoters. Mol Plant Microbe Interact. 1993 May-Jun;6(3):323–330. doi: 10.1094/mpmi-6-323. [DOI] [PubMed] [Google Scholar]
  42. Reusken C. B., Neeleman L., Bol J. F. The 3'-untranslated region of alfalfa mosaic virus RNA 3 contains at least two independent binding sites for viral coat protein. Nucleic Acids Res. 1994 Apr 25;22(8):1346–1353. doi: 10.1093/nar/22.8.1346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Rubino L., Russo M. Characterization of resistance to cymbidium ringspot virus in transgenic plants expressing a full-length viral replicase gene. Virology. 1995 Sep 10;212(1):240–243. doi: 10.1006/viro.1995.1476. [DOI] [PubMed] [Google Scholar]
  44. Schiebel W., Haas B., Marinković S., Klanner A., Sänger H. L. RNA-directed RNA polymerase from tomato leaves. II. Catalytic in vitro properties. J Biol Chem. 1993 Jun 5;268(16):11858–11867. [PubMed] [Google Scholar]
  45. Sit T. L., Leclerc D., AbouHaidar M. G. The minimal 5' sequence for in vitro initiation of papaya mosaic potexvirus assembly. Virology. 1994 Feb 15;199(1):238–242. doi: 10.1006/viro.1994.1118. [DOI] [PubMed] [Google Scholar]
  46. Smith H. A., Swaney S. L., Parks T. D., Wernsman E. A., Dougherty W. G. Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs. Plant Cell. 1994 Oct;6(10):1441–1453. doi: 10.1105/tpc.6.10.1441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Stanley J., Frischmuth T., Ellwood S. Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6291–6295. doi: 10.1073/pnas.87.16.6291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Suzuki M., Masuta C., Takanami Y., Kuwata S. Resistance against cucumber mosaic virus in plants expressing the viral replicon. FEBS Lett. 1996 Jan 22;379(1):26–30. doi: 10.1016/0014-5793(95)01477-2. [DOI] [PubMed] [Google Scholar]
  49. Taschner P. E., van Marle G., Brederode F. T., Tumer N. E., Bol J. F. Plants transformed with a mutant alfalfa mosaic virus coat protein gene are resistant to the mutant but not to wild-type virus. Virology. 1994 Sep;203(2):269–276. doi: 10.1006/viro.1994.1484. [DOI] [PubMed] [Google Scholar]
  50. Tenllado F., García-Luque I., Serra M. T., Díaz-Ruíz J. R. Resistance to pepper mild mottle tobamovirus conferred by the 54-kDa gene sequence in transgenic plants does not require expression of the wild-type 54-kDa protein. Virology. 1996 May 1;219(1):330–335. doi: 10.1006/viro.1996.0257. [DOI] [PubMed] [Google Scholar]
  51. Wassenegger M., Heimes S., Riedel L., Sänger H. L. RNA-directed de novo methylation of genomic sequences in plants. Cell. 1994 Feb 11;76(3):567–576. doi: 10.1016/0092-8674(94)90119-8. [DOI] [PubMed] [Google Scholar]
  52. Wisniewski L. A., Powell P. A., Nelson R. S., Beachy R. N. Local and systemic spread of tobacco mosaic virus in transgenic tobacco. Plant Cell. 1990 Jun;2(6):559–567. doi: 10.1105/tpc.2.6.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Yamaya J., Yoshioka M., Meshi T., Okada Y., Ohno T. Expression of tobacco mosaic virus RNA in transgenic plants. Mol Gen Genet. 1988 Mar;211(3):520–525. doi: 10.1007/BF00425710. [DOI] [PubMed] [Google Scholar]
  54. Zaccomer B., Cellier F., Boyer J. C., Haenni A. L., Tepfer M. Transgenic plants that express genes including the 3' untranslated region of the turnip yellow mosaic virus (TYMV) genome are partially protected against TYMV infection. Gene. 1993 Dec 22;136(1-2):87–94. doi: 10.1016/0378-1119(93)90451-8. [DOI] [PubMed] [Google Scholar]
  55. Zaitlin M., Anderson J. M., Perry K. L., Zhang L., Palukaitis P. Specificity of replicase-mediated resistance to cucumber mosaic virus. Virology. 1994 Jun;201(2):200–205. doi: 10.1006/viro.1994.1286. [DOI] [PubMed] [Google Scholar]
  56. Ziegler-Graff V., Guilford P. J., Baulcombe D. C. Tobacco rattle virus RNA-1 29K gene product potentiates viral movement and also affects symptom induction in tobacco. Virology. 1991 May;182(1):145–155. doi: 10.1016/0042-6822(91)90658-x. [DOI] [PubMed] [Google Scholar]
  57. de Carvalho Niebel F., Frendo P., Van Montagu M., Cornelissen M. Post-transcriptional cosuppression of beta-1,3-glucanase genes does not affect accumulation of transgene nuclear mRNA. Plant Cell. 1995 Mar;7(3):347–358. doi: 10.1105/tpc.7.3.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. de Feyter R., Young M., Schroeder K., Dennis E. S., Gerlach W. A ribozyme gene and an antisense gene are equally effective in conferring resistance to tobacco mosaic virus on transgenic tobacco. Mol Gen Genet. 1996 Feb 25;250(3):329–338. doi: 10.1007/BF02174391. [DOI] [PubMed] [Google Scholar]
  59. de Haan P., Gielen J. J., Prins M., Wijkamp I. G., van Schepen A., Peters D., van Grinsven M. Q., Goldbach R. Characterization of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants. Biotechnology (N Y) 1992 Oct;10(10):1133–1137. doi: 10.1038/nbt1092-1133. [DOI] [PubMed] [Google Scholar]

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