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. 2005 Dec;167(6):1713–1728. doi: 10.1016/S0002-9440(10)61253-9

Figure 5.

Figure 5

Immunohistochemical staining of LC3 in brain tissues of CD−/− and CB−/−CL−/− mice and their littermate controls. A and B: Cerebral cortical regions of CD+/− (A) and CD−/− (B) mouse brains at P24. Positive staining of LC3 appears fibrillary in dendrites of pyramidal neurons and diffuse in their perikarya (A, inset). Its staining is also fibrillary in dendrites of the neurons but appears granular in their perikarya (B, inset). C and D: Purkinje cells in the cerebellum of CB+/−CL+/− (C) and CB−/−CL−/− (D) mice at P12. Fibrillary staining of LC3 is demonstrated in dendrites of Purkinje cells from both control and mutant mice (C, D), whereas its staining is relatively weak and diffuse in the perikarya of CB+/−CL+/− control neurons (C, inset) but intense and granular in those of CB−/−CL−/− neurons (D, inset). E and F: Nerve fibers in the corpus callosum of CB+/−CL+/− (E) and CB−/−CL−/− (F) mice at P12. No clear-cut staining for LC3 can be seen in the control axons (E), whereas intensely stained dots are accumulated in the mutant axons. Scale bars: 50 μm (A–F); 20 μm (insets in A–D).