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TNF-α and Th2-derived factors induce adhesion molecule expression on bEnd.3 cells. Confluent bEnd.3 cells were either left unstimulated (dotted line) or stimulated (solid line) for 6 hours with TNF-α (10 ng/ml) or Th2 supernatant prepared as described in Materials and Methods. Endothelial cells were briefly trypsinized (<5minutes), stained with monoclonal antibodies for the indicated adhesion molecules, and analyzed by flow cytometry. Histograms represent 30,000 events.
