Figure 6.

EHEC reduces epithelial tight junction protein expression. A: Representative immunoblots for tight junction proteins in protein extracts from T84 cell monolayers 16 hours after EHEC infection (arrows indicate reduced expression; representative of six to nine monolayers from three experiments; doublets in occludin and ZO-1 immunoblots likely indicate phosphorylated and nonphosphorylated forms). B: Claudin-2 is significantly reduced by comparison of claudin-2:claudin-4 ratio in five experiments (each symbol indicates the average of three epithelial preparations/experiment).