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Expression of viral HAs. (A) The structure of the vectors, together with the indicated specific mutations in the cleavage site, for immunogens and lentiviral vector pseudotypes. (B) Expression of the indicated viral HAs was determined by Western blot analysis with antisera reactive to the 1918 influenza HA. Expression was evaluated after transfection of the indicated plasmids in 293T cells. Arrows indicate the relevant viral HA bands.
