Table 3.
Arginine sensitivity of rat hepatic cells for known stress response-linked genes.
| A. Normal Hepatic Cells | ||||
| Annotation | Gene name | Affymetrix ID | Change | P value |
| Metabolism (Cholesterol biosynthesis) | farensyl diphosphate synthase | 1370808_at | ↓ | 0.00002 |
| farnesyl diphosphate farnesyl transferase 1 | 1387119_at | ↓ | 0.00011 | |
| 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 | 1373243_at | ↓ | 0.00002 | |
| cytochrome P450, subfamily 51 | 1368232_at | ↓ | 0.00002 | |
| mevalonate pyrophosphate decarboxylase | 1375852_at | ↓ | 0.00043 | |
| 7-dehydrocholesterol reductase | 1386990_at | ↓ | 0.00013 | |
| mevalonate kinase | 1388218_at | ↓ | 0.00002 | |
| isopentenyl-diphosphate delta isomerase | 1367839_at | ↓ | 0.00002 | |
| diaphorase 1 | 1372012_at | ↓ | 0.00077 | |
| Sterol 14 alpha-demethylase (CYP51) | 1387020_at | ↓ | 0.00002 | |
| 3-hydroxy-3-methylglutaryl-Coenzyme A reductase | 1367932_at | ↓ | 0.00002 | |
| phenylalkylamine Ca2+ antagonist (emopamil) binding protein | 1367667_at | ↓ | 0.00021 | |
| cytochrome P450, subfamily 51 | 1368189_at | ↓ | 0.00002 | |
| mevalonate kinase | 1368878_at | ↓ | 0.00120 | |
| low density lipoprotein receptor | 1388872_at | ↓ | 0.00021 | |
| isopentenyl-diphosphate delta isomerase | 1368020_at | ↓ | 0.00002 | |
| Metabolism and energy pathways | ATP citrate lyase | 1367854_at | ↓ | 0.00002 |
| Metabolism (Urea Cycle) | arginase 1 | 1368266_at | ↓ | 0.000492 |
| glutamate dehydrogenase 1 | 1370200_at | ↓ | 0.00002 | |
| heterogeneous nuclear ribonucleoprotein A/B | 1367754_s_at | ↓ | 0.001651 | |
| ornithine aminotransferase | 1367729_at | ↓ | 0.002753 | |
| argininosuccinate lyase | 1368916_at | ↓ | 0.015426 | |
| Growth arrest and DNA damage inducible gene | Gadd45g-predicted | 1388792_at | ↓ | 0.00024 |
| Gadd45b-predicted | 1372016_at | ↓ | 0.00778 | |
| Gadd45gip_1predicted | 1371896_at | ↓ | 0.00077 | |
| Stress Response | growth response protein (CL-6) | 1367894_at | ↓ | 0.00002 |
| voltage-dependent anion channel 1 | 1386909_a_at | ↓ | 0.00107 | |
| Apoptosis | ankyrin-like repeat protein | 1367664_at | ↓ | 0.00005 |
| B. Hepatic Tumor Cells | ||||
| Annotation | Gene name | Affymetrix ID | Change | P value |
| Metabolism (Cholesterol biosynthesis) | farnesyl diphosphate farnesyl transferase 1 | 1367839_at | ↓ | 0.00110 |
| 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 | 1367932_at | ↓ | 0.00002 | |
| cytochrome P450, subfamily 51 | 1367979_s_at | ↓ | 0.00014 | |
| 7-dehydrocholesterol reductase | 1368189_at | ↓ | 0.00043 | |
| isopentenyl-diphosphate delta isomerase | 1368878_at | ↓ | 0.00004 | |
| protein kinase, AMP-activated, alpha 1 catalytic subunit | 1369104_at | ↓ | 0.05055 | |
| diaphorase 1 | 1370808_at | ↓ | 0.00304 | |
| Dhcr24-predicted | 1372012_at | ↓ | 0.01202 | |
| phenylalkylamine Ca2+ antagonist (emopamil) binding protein | 1386990_at | ↓ | 0.00165 | |
| cytochrome P450, subfamily 51 | 1387020_at | ↓ | 0.00650 | |
| low density lipoprotein receptor | 1388218_at | ↓ | 0.00002 | |
| mevalonate pyrophosphate decarboxylase | 1368020_at | ↑ | 0.03578 | |
| Metabolism and energy pathways | ATP citrate lyase | 1367854_at | ↓ | 0.00002 |
| Metabolism (Urea Cycle) | glutamate dehydrogenase 1 | 1370200_at | ↓ | 0.00359 |
| glutamate dehydrogenase 1 | 1387878_at | ↓ | 0.00021 | |
| ornithine aminotransferase | 1367729_at | ↓ | 0.00002 | |
| arginase 1 | 1368266_at | ↓ | 0.00061 | |
| Growth arrest & DNA damage inducible gene | Gadd45g-predicted | 1388792_at | ↓ | 0.00249 |
| Gadd45gip_1predicted | 1371896_at | ↓ | 0.00133 | |
| Stress Response | oxygen regulated protein (150 kD) | 1370665_at | ↓ | 0.00141 |
| solute carrier family 2, (facilitated glucose transporter) member 8 | 1368286_at | ↓ | 0.00049 | |
| growth response protein (CL-6) | 1367894_at | ↓ | 0.00004 | |
| insulin induced gene 2 | 1389377_at | ↑ | 0.02475 | |
| protease, serine, 25 | 1367478_at | ↑ | 0.00004 | |
| Apoptosis | ankyrin-like repeat protein | 1367664_at | ↓ | 0.01202 |
| glycogen synthase kinase 3 beta | 1370267_at | ↓ | 0.00249 | |
| valosin-containing protein | 1367455_at | ↓ | 0.03841 | |
Genes were selected based on key word categories/annotations shown and search of Affymetrix rat gene chip probes. Not all genes associated with stress response in human cells are included or identifiable in the rat gene chip. Genes scoring as absent in all 6 normal or tumor samples were eliminated from further analysis. For genes scoring as present, the average value of (+) arginine and (-) arginine was calculated for three replicate samples. Direction of change in expression upon arginine deprivation and the associated p value for such change is presented.