Skip to main content
. Author manuscript; available in PMC: 2006 Oct 20.
Published in final edited form as: J Biol Chem. 2006 Aug 18;281(42):32004–32014. doi: 10.1074/jbc.M606876200

Fig. 3. PLM associates with proximal and distal regions of intracellular loop by GST pulldown.

Fig. 3

GST pulldown assays were performed as described in Fig. 2, except that the GST-NCX1 fusion proteins contained varying segments of the intracellular loop of NCX1 and that bacterial derived His-tagged PLM (28) was used. Upper panel: GST and GST-NCX1 fusion proteins detected by anti-GST antibody. Arrows (in descending order) point to the predicted mw of GST-NCX1/218-764 (81 kDa), GST-NCX1/508-764 (52 kDa), GST-NCX1/218-371 (41 kDa), GST-NCX1/371-508 (40 kDa) and GST (26 kDa). Lower panel: His-tagged PLM detected by anti-His antibody. This experiment was performed 4 times with similar results.