Figure 7.

Deletion of Prmp C terminus reduces process length of CG4 cells. CG4 cells were transfected with an expression vector containing Prmp tagged with Myc (A). Cotransfection with EGFP was used to measure the length of the longest process (B). A C-terminal deletion mutant (Prmp-ct) was transfected into CG4 cells (C). The corresponding EGFP channel in C demonstrates the severe reduction in process length of CG4 cells that have been transfected with Prmp-ct (D). E, Control EGFP transfected CG4 cells. DAPI staining of nuclei is blue. Scale bar, 10 μm. F, Quantitation of process length after Prmp transfection in CG4 cells. The longest process was measured from the cell body to the end of the process using the EGFP channel and IPLab software. Fifty cells from two independent experiments were measured for each group. Error bars represent SEM. *p < 0.01, Student's t test (Prmp-wt compared with Prmp-ct).