Table 3.
Combinations of Antibodies Used for Double/Triple Immunofluorescence on Hodgkin’s Lymphoma
| Green fluorescence* | Red fluorescence† | Blue fluorescence‡ | Coexpression in H/RS cells |
|---|---|---|---|
| HPC1 | BMI-1 | Ki67 | Yes |
| HPC2 | BMI-1 | Ki67 | Yes |
| HPH1 | BMI-1 | Ki67 | Yes |
| RING1 | BMI-1 | Ki67 | Yes |
| EZH2 | BMI-1 | Ki67 | Yes |
| CtBP | BMI-1 | Ki67 | Yes |
| MEL-18 | BMI-1 | Yes | |
| HPC1 | BMI-1 | EED | Yes |
| HPC2 | BMI-1 | EED | Yes |
| HPH1 | BMI-1 | EED | Yes |
| RING1 | BMI-1 | EED | Yes |
| EZH2 | BMI-1 | EED | Yes |
| CtBP | BMI-1 | EED | Yes |
| MEL-18 | Ki67 | Yes |
*
Polyclonal rabbit-derived antibodies were visualized using an ALEXA 488-conjugated goat-anti-rabbit antiserum.
†
Antibodies with an IgG2b subtype were visualized with an HRP-conjugated goat-anti-mouse IgG2b specific antibody followed by rhodamine/tyramine intensification.
‡
Antibodies if IgG1 or IgG2a subtype were detected by a biotinylated goat-anti-mouse IgG1 or IgG2a followed by allophycocyanin-coupled streptavidine.