RAF profiles amplified from sugarcane (a), tomato and soybean (b), and wheat (c). Radio-labelled profiles amplified from replicate ((a) and (b)) or triplicate (c) genomic DNA extractions were run side-by-side on the gel and given the same lane number. The Operon oligonucleotide primers used are also listed above the lanes. (a) RAF profiles amplified from different amounts of genomic DNA extracted from sugarcane hybrid 79A362. Lanes 1, 12.5 ng; lanes 2, 25 ng; lanes 3, 50 ng; lanes 4, 100 ng; lanes 5, 250 ng; and lanes 6, 500 ng. (b) RAF profiles amplified from tomato cv. Moneymaker (lanes 1), Glycine soja (lanes 2), soybean (G. max) cv. Bragg (lanes 3). (c) RAF profiles amplified from wheat cv. Hartog; lanes 1-5 represent individual Hartog plants.