Skip to main content
. 2002;2(3):141–150. doi: 10.1155/S1110724302206026

Figure 5.

Figure 5

Selective amplification of RAF markers is dependent on the 3′ nucleotide of the oligonucleotide primer. (a) Radio-labelled RAF profiles for sugarcane hybrid 79A362 using oligonucleotide primers K-02 (5′-GTCTCCGCAA-3′) (lanes A), K-02a (5′-GTCTCCGCAC-3′) (lanes C), K-02b (5′-GTCTCCGCAG-3′) (lanes G), and K-02c (5′-GTCTCCGCAT-3′) (lanes T). These primers only varied in the final 3′ nucleotide. The profiles were amplified from replicate DNA extractions of sugarcane 79A362. (b) Identical reactions, also in replicate, were produced without radio-labelling, separated on a 2% agarose gel, ethidium-bromide stained, and photographed. (c) The unlabelled RAF fragments were then blotted from the agarose gel onto a nylon membrane and probed with radio-labelled RAF products amplified with K-02 (lanes A in panel (a)).