Figure 2.

Perillyl alcohol, but not d-limonene, inhibits breast cell migration. MCF-10A cells were allowed to migrate towards the bottom side of an 8- μm pore filter coated with fibronectin for 18 hours in serum-free minimal medium containing the indicated concentrations of monoterpenes, or serum as a control. Migration towards nonfat dried milk (blotto) served as a negative control for random migration. Migration was quantitated by fluorescently labeling cells on the bottom of the filters and reading the fluorescence with a plate reader. Results expressed as mean of Relative Fluorescence Units, ± standard deviation (n = 3). A student-Numan-Keuls test identified five groups (letters A-E; P ≤ .05) which were statistically different from one another (ANOVA F:[7, 23] = 231.76, P < .0001).