FIG. 2.

PUT gene expression after treatment with rapamycin. Protease-deficient strain BJ2168 carrying plasmids pYEp13 (LEU2) and either pWB36 (PUT1-lacZ) or pABC4 (PUT2-lacZ) was grown in minimal glucose-glutamine medium supplemented with tryptophan. Rapamycin (+; 200 ng/ml) or the drug vehicle alone (−; 90% ethanol-10% Tween 20) was added, and the cells were harvested 60 min later and assayed for β-galactosidase activity. Results are the average of three independent experiments with standard deviation as indicated.