TABLE 7.
SPB fluorescence of GFP fusion proteins in MOP mutants
| Relevant genotype | Relative intensity of GFP foci (% of signal from wild type)a with the following GFP fusion:
|
|||
|---|---|---|---|---|
| Ady4p | Mpc54p | Mpc70p | Spo74p | |
| ady4Δ/ady4Δ | NDb | 94 ± 21 | 100 ± 49 | 93 ± 57 |
| cnm67Δ/cnm67Δ | —c | ND | ND | — |
| mpc54Δ/mpc54Δ | 16 ± 32 | ND | 46 ± 28 | 39 ± 43 |
| mpc70Δ/mpc70Δ | 4 ± 12 | 42 ± 23 | ND | — |
| spo74Δ/spo74Δ | <1 ± 4 | 18 ± 16 | — | ND |
a
Fluorescent signals (mean ± standard deviation) were quantified as described in Materials and Methods.
b
ND, not determined.
c
—, no fluorescent signal at SPBs.