TABLE 1.
Fatty acid composition in total lipids of Ax2, mfeA-null cells, and E. colia
| Fatty acid | Composition in:
|
||||
|---|---|---|---|---|---|
| E. coli | Wild type
|
mfeA−
|
|||
| E. coli (%) | HL5 (%) | E. coli (%) | HL5 (%) | ||
| C16:0 | 32.7 | 5.1 | 6.3 | 7.4 | 8.0 |
| C16:1(9) | 4.2 | 2.3 | 4.0 | 6.3 | 7.5 |
| C16:2(5,9) | 5.4 | 1.7 | 4.9 | 3.7 | |
| C18:1(11) | 23.8 | 21.8 | 31.8 | 18.5 | 29.2 |
| C18:2(5,9) | 2.9 | 15.2 | 1.1 | 11.1 | |
| C18:2(5,11) | 41.4 | 32.7 | 17.2 | 14.0 | |
| cy17:0 | 19.5 | 7.6 | 20.7 | ||
| cy19:0 | 13.0 | 8.9 | 15.0 | ||
a
Fatty acid composition was analyzed for the total lipids extracted from E. coli and Dictyostelium cells that had been grown for 24 h on an E. coli lawn as described in Materials and Methods. Also, it was notable that the ratio of polyunsaturated fatty acids increased to 6.1% in axenically grown mfeA-null cells. In addition to these major fatty acids, C14:0, C16:1(5), and C18:0 were detected as minor components that constituted less than 5% of total fatty acids of bacterially grown wild-type and mfeA-null cells.