FIG. 4.

The glucose phosphorylation mutant releases glucose into the culture medium via the secretory pathway. (A) Amount of glucose released into the culture medium by the indicated strains grown in YPGal medium or YPGal supplemented with 1 mM 1-deoxynojirimycin and 1 mM castanospermine (Dno/Csp). Cultures were grown for 36 h. (B) Concentrations of glucose accumulated in cells after BFA treatment. Cultures of the wild-type and glucose phosphorylation mutant strains grown in YPGal medium were divided into two aliquots. BFA (224 μg/ml) was added to one aliquot from each strain, and incubation was continued under the same culture conditions for 4.5 h before the cells were harvested and the cellular glucose levels were determined.