FIG. 1.
(A) Domain structure of Sql2 and its homologues. Domains indicated below were identified using SMART and CDART (http://smart.embl-heidelberg.de/ and http://www.ncbi.nlm.nih.gov/Structure/lexington/lexington.cgi?cmd=rps). Domain annotations: SH3, pfam0001; RasGEFN, smart00229; and RasGRF_CDC25, IPR001895. Numbers represent the amino acid (aa) positions of each domain. Accession numbers: Sql2, AY149915; Csc25p, S30356; Cdc25p, CAA27259; Sdcp, P14771; Ste6, P26674; and Efc25, T40241. (B) Overexpression of sql2 with the otef promoter. Strains indicated at the top were incubated in PD liquid medium; 15 μg of total RNA was loaded per lane. The same filter was hybridized in succession with probes for sql2 and cbx as loading control. (C) Overexpression of sql2 suppresses the glossy colony appearance of a gpa3Q206L strain. FB1 derivatives indicated at the top were grown on PD-charcoal plates (upper panel) or in PD liquid medium (lower panel). Bars, 1 mm (upper panel) and 20 μm (lower panel).