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Dominant-active ras1Q67L elevates pheromone gene expression. Strains indicated at the top were incubated in CM containing glucose (−) and then for 5 h in arabinose (+) as the carbon source. A 15-μg portion of total RNA was loaded per lane. The same filter was hybridized in succession with probes for mfa1, frb34, and rRNA as the loading control.
