FIG. 2.

Disruption of tmkA by homologous integration. (A) Strategy for single-crossover integration. Map positions are labeled in base pairs, starting with the first BamHI site. (B) Strategy for double-crossover integration. Map positions are labeled in base pairs, starting with the first BamHI site. (C) Southern analysis of transformants. Genomic DNA was digested with the indicated enzymes, and the blot was probed with the fragment labeled MKG1 in panel A. WT, wild type. (D) RT-PCR analysis of transformants. No products were detected when RT was omitted from the reactions (right lanes).