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Measurement of the asymmetry parameter Asym along a vesicle trajectory. To estimate Asym for a given point of the trajectory (indicated by the arrowhead), this parameter was calculated for various window widths (marked in bold) centered on the point of interest. Only the window width that maximized Asym value was retained (boxed). The asymmetry profile was obtained by repeating this procedure point by point along the trajectory (see Fig. 4).
