TABLE 1.
Effect of different nitrogen sources on branching frequency in C. albicans hyphae
| Treatment | Supplements | No. of hyphal compartments before first brancha |
|---|---|---|
| Control (0.5% [vol/vol] serum) | 5.9 ± 0.2 | |
| Amino acids | Val-Leu-Ile | 5.1 ± 0.2 |
| Gly-Ala-Pro | 3.4 ± 0.1 | |
| Ser-Met-Thr | 4.0 ± 0.2 | |
| Cys-Asn-Gln | 3.5 ± 0.1 | |
| Phe-Tyr-Trp | 5.3 ± 0.3 | |
| His-Arg-His | 3.7 ± 0.1 | |
| Asp-Glu | 3.8 ± 0.1 | |
| Trypsinb | 3.4 ± 0.2 | |
| Heat-inactivated trypsin | 5.4 ± 0.5 |
a
Mean number of compartments from the hyphal apex to the first branch in 0.5% (vol/vol) serum agar with mixtures of various nitrogen supplements. Values are means ± standard errors (n = 14) from two independent experiments. The standard error for the control is from seven independent experiments (n = 49), and the trypsin results are based on a single experiment (n = 15).
b
Newborn calf serum (0.5%) was digested with 10 μg of trypsin per ml for 16 h at 37°C and then heat-inactivated (76).